ab 2636961  (Cell Signaling Technology Inc)

Journal: Endocrinology

Article Title: Roles of Growth Hormone-Dependent JAK-STAT5 and Lyn Kinase Signaling in Determining Lifespan and Cancer Incidence.

doi: 10.1210/endocr/bqae136

Figure Lengend Snippet: Figure 3. Altered mitochondrial biogenesis regulator and sirtuins transcripts in liver of 12-week-old Ghr-mutant mice fed ad libitum. (A) Mitochondrial biogenesis regulator Pgc1α transcripts were significantly elevated in Ghr mutants while Pgc1β was upregulated only in Ghr male mutants. (B) Key mitochondrial sirtuin, Sirt3 transcript was elevated in all the mutants independent of sex while Sirt1 and 2 were elevated mostly in Ghr−/− males and Sirt6 in Ghr−/− and Ghr391−/− males. Data represented as mean ± SEM (n = 4 mice/genotype). *P less than .05; **P less than .01; ***P less than .001; ****P less than .0001 compared to wild-type (WT) of the same sex by analysis of variance.

Article Snippet: PGC1 ab106814 AB 10865490 Abcam Total Oxphos Rodent Antibody ab110413 AB 2629281 Abcam SIRT1 (DID7) 9475 AB 2617130 Cell Signaling Technology SIRT2 (D4S6J) 12672 AB 2636961 Cell Signaling Technology SIRT3 (D22A3) 5490 AB 10696895 Cell Signaling Technology SIRT6 (D8D12) 12486 AB 2636969 Cell Signaling Technology P-p38 MAPK (Thr180/Tyr182) 4511 AB 2139682 Cell Signaling Technology p38 MAPK 9212 AB 330713 Cell Signaling Technology SDHB (E3H9Z) 92649 AB 3644290 Cell Signaling Technology COX IV 4844 AB 2085427 Cell Signaling Technology

Techniques: Mutagenesis

Journal: Endocrinology

Article Title: Roles of Growth Hormone-Dependent JAK-STAT5 and Lyn Kinase Signaling in Determining Lifespan and Cancer Incidence.

doi: 10.1210/endocr/bqae136

Figure Lengend Snippet: Figure 4. Protein levels of mitochondrial and key sirtuins in liver of 12-week-old male Ghr-mutant mice fed ad libitum. (A) Increase in protein level of Pgc1, Sirt1 and 3 in Ghr−/− and GhrBox1−/− mutants. No change in Sirt2 isoforms was seen, whereas a decrease in Sirt 6 was observed in GhrBox1−/−. (B) Blots representative of n = 3 mice/genotype. Elevated Sirt3 protein in Ghr391−/− male mutant mice as compared to wild-type (WT). Blots representative of n = 3 mice/genotype.

Article Snippet: PGC1 ab106814 AB 10865490 Abcam Total Oxphos Rodent Antibody ab110413 AB 2629281 Abcam SIRT1 (DID7) 9475 AB 2617130 Cell Signaling Technology SIRT2 (D4S6J) 12672 AB 2636961 Cell Signaling Technology SIRT3 (D22A3) 5490 AB 10696895 Cell Signaling Technology SIRT6 (D8D12) 12486 AB 2636969 Cell Signaling Technology P-p38 MAPK (Thr180/Tyr182) 4511 AB 2139682 Cell Signaling Technology p38 MAPK 9212 AB 330713 Cell Signaling Technology SDHB (E3H9Z) 92649 AB 3644290 Cell Signaling Technology COX IV 4844 AB 2085427 Cell Signaling Technology

Techniques: Mutagenesis